About Me

PhD

I was awarded a doctorate in biochemistry/biophysics at the University of Sydney under the supervision of Professor Anthony Weiss. My project aimed to further the current understanding of the elastin precursor tropoelastin using full-length recombinant human tropoelastin as well as smaller constructs of the molecule and peptides based on individual domains. I have a broad skills base that includes both molecular biology and structural biophysics techniques.

Methods

Matrix Assisted Laser Desorption Ionisation Mass Spectrometry (MALDI MS)
Small angle X-ray scattering (SAXS)
Light scattering
Circular Dichroism
Vibrational spectroscopy (FTIR and Raman)
Protein expression and purification
Chromatography- gel filtration and reverse phase HPLC
High throughput microtitre plate assays
Luminsecence
Fluorescence
Mammalian cell transfection
Mammalian cell culture and cell handling (HEK cells)
Purification of Plasmid DNA
Primer Design, PCR and mutagenesis
Preparation and transformation of competent bacteria
Electrophoresis (glycine and tricine polyacryamide gels and agarose gels)
Western blots
Tensile testing